A deranged German scientist mutilates the bodies of a group of people to reassemble them into one body, so Lindsay, the girl coming from the United States with her friend, becomes his final victim.
As a mentally unstable man grows increasingly fascinated by the experiment conducted by Dr. Heiter in the horror movie The Human Centipede (First Sequence), he attempts to create a human centipede of his own.
In an attempt to get control over the unruly inmates, a disturbed prison warden takes inspiration from The Human Centipede films and mutilates the prisoners to create a 500-person human centipede.