| Name | Role/Job | Order of appearance | Options | |
|---|---|---|---|---|
| 1) | Bill Murray | Frank | 1 | |
| 2) | Molly Shannon | Mrs. Boyd | 2 | |
| 3) | Chris Elliott | Bob | 3 | |
| 4) | Elena Franklin | Shane | 4 | |
| 5) | Chris Rock | Osmosis Jones | 5 | |
| 6) | Laurence Fishburne | Thrax | 6 | |
| 7) | David Hyde Pierce | Drix (voice) | 7 | |
| 8) | Brandy Norwood | Leah | 8 | |
| 9) | William Shatner | The Mayor | 9 | |
| 10) | Danny Murphy | Zookeeper Superintendent | 10 | |
| 11) | Malin Akerman | Kidney Rock | 11 | |
| 12) | Ron Howard | Tom Colonic | 12 | |
| 13) | Grady O'Connor | Zoo Visitors & Staff | 18 | |
| 14) | Danny Mann | 19 | ||
| 15) | Mark Duplass | Science Fair Contestant | 21 | |
| 16) | Ruth Meyer | Zoo Visitors & Staff | 29 | |
| 17) | Jack McBrayer | Zoo Visitors & Staff | 35 | |
| 18) | Jo Ann Nicewicz | Docky's Clamshack | 38 | |
| 19) | Jack McCullough | Zookeeper | 42 | |
| 20) | Anne Lockhart | 43 | ||
| 21) | Nikki Tyler-Flynn | Nurse #2 | 44 | |
| 22) | Paul Tuerpe | 45 | ||
| 23) | Robert Wisdom | 56 | ||
| 24) | Chandler Vuilleumier | Volcano Gal | 57 | |
| 25) | Mattie Ostiguy | Emergency Room Nurse | 62 | |
| 26) | Chris Phillips | 66 | ||
| 27) | Lynne Redding | 67 | ||
| 28) | Paul Pape | 68 | ||
| 29) | Zak Penn | Emergency Room Doctor #3 | 69 | |
| 30) | Marilyn Pasekoff | 70 | ||
| 31) | Amy Suhay | Bikini Gang | 72 | |
| 32) | Dick Silva | Science Fair Contestant | 73 | |
| 33) | Herschel Sparber | 75 | ||
| 34) | Sydney J. Bartholomew Jr | 82 | ||
| 35) | Richard Steven Horvitz | 83 | ||
| 36) | Judy Conrad | Zoo Visitors & Staff | 86 | |
| 37) | Donald Fullilove | 99 | ||
| 38) | Zen Gesner | Emergency Room Doctor #1 | 106 | |
| 39) | Eddie Frierson | 107 | ||
| 40) | Sean Fitzgerald | Police Officer | 108 | |
| 41) | Carlos Alazraqui | 109 | ||
| 42) | Marc Hyman | Emergency Room Doctor #2 | 111 | |
| 43) | Keith Anthony | 112 | ||
| 44) | Sally Ann Brooks | 119 | ||
| 45) | Abe Farrelly | Bus Girl #2 | 123 | |
| 46) | Janis Dardaris | 127 | ||
| 47) | Catherine Dunphy | Volcano Gal | 130 | |
| 48) | Stefanie Eulinberg | 131 | ||
| 49) | Jackie Flynn | School Janitor | 134 | |
| 50) | Kena Flynn | Science Fair Contestant | 135 | |
| 51) | Maryjane Foy | Docky's Clamshack | 137 | |
| 52) | Jonathan Adams | 162 |
| Name | Role/Job | Order of appearance | Options | |
|---|---|---|---|---|
| 1) | Bobby Farrelly | Producer | 4 | |
| 2) | Peter Farrelly | Producer | 5 | |
| 3) | Zak Penn | Producer | 6 |
| Name | Role/Job | Order of appearance | Options | |
|---|---|---|---|---|
| 1) | Bobby Farrelly | Director | 1 | |
| 2) | Peter Farrelly | Director | 2 |
| Name | Role/Job | Order of appearance | Options | |
|---|---|---|---|---|
| 1) | Lois Freeman-Fox | Editor | 11 | |
| 2) | Sam Seig | Editor | 13 |
| Name | Role/Job | Order of appearance | Options | |
|---|---|---|---|---|
| 1) | Sydney J. Bartholomew Jr | 1 |
| Name | Role/Job | Order of appearance | Options | |
|---|---|---|---|---|
| 1) | Marc Hyman | Screenplay | 1 |
| Name | Role/Job | Order of appearance | Options | |
|---|---|---|---|---|
| 1) | Randy Edelman | Original Music Composer | 8 |
| Name | Role/Job | Order of appearance | Options | |
|---|---|---|---|---|
| 1) | Mark Irwin | Director of Photography | 10 |
| Name | Synopses | Official ? | Options |
|---|---|---|---|
| Basel | Frank Detumilo, a zoo guard, is the father of a beautiful girl named Shane. A germ sneaks into Frank's body. Osmosis Jones is guarding Frank, in a group of white blood cells. Jones discovers that the effect of the bacterium on Frank leads to death, after being infected with a deadly virus known as Thrax. The Thrax virus makes a tight plan to kill Frank, while Jones tries to save Frank's life. 395 |
| Name | Review Content | Spoiler ? | Official ? | Options |
|---|